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In Haemophilus influenzae, competence and transformation are tightly controlled. This control is facilitated by regulating expression of the genes in the competence regulon from a site upstream of each competence operon. This site is known as the Competence Regulatory Element (CRE) and it is a highly conserved sequence. Ten possible CRE sites were known to exist in the genome of H. influenzae. Putative CRE regions in H. influenzae Rd KW20. A. Organization of CDSs contiguous to the putative CRE elements in the Rd KW20 genome. CRE numbers assigned are based upon the original designation of the predicted targeted gene. Genes shaded black indicate biological data confirming their role in transformation in H. influenzae. Genes shaded grey indicate mutations affecting transformation exist in the operon but polar effects prevent individual characterization. CRE0937 was originally designated as a CRP binding site. Putative terminators are designated t. B. Comparison of the putative CRE sequences in the Rd KW20 genome and association with transformation in H. influenzae. ND indicates role undetermined.
We essentially knew that 5 of these ten possible CRE controlled operons had genes in them that were involved in competence and transformation. What about the other five?
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